Signalling

Part:BBa_K511300:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)


Notch-Gal4-ESN Juxtacrine Signaling Receptor MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
    Illegal NotI site found at 5216
    Illegal NotI site found at 5224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2925
    Illegal XhoI site found at 5507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
    Illegal NgoMIV site found at 516
    Illegal NgoMIV site found at 2628
    Illegal NgoMIV site found at 2684
    Illegal NgoMIV site found at 2727
    Illegal NgoMIV site found at 3512
    Illegal NgoMIV site found at 4529
    Illegal NgoMIV site found at 4706
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5426
    Illegal BsaI.rc site found at 85
    Illegal BsaI.rc site found at 1793
    Illegal BsaI.rc site found at 2708
    Illegal BsaI.rc site found at 5165


Design Notes

Because of the substantial size of the Notch-Gal4-ESN part (roughly 6000 base pairs), it is recommended that internal primers are designed for sequencing of constructs including this part. Additionally, we have noted some difficulty in producing Notch-Gal4 through polymerase chain reaction (PCR) methods. We recommend using 5% DMSO to produce this part via PCR.


Source

This part is derived from the human Notch-1 receptor and the Gal4 transactivation protein from Saccharomyces cerevisiae.

References